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2c), suggesting that synergistic action on Il12rb2 expression with TCR activation and cAMP was mediated by IL 2 and this phenomenon did not appear until 24h of stimulation. Consistently, blocking either IFN or IL 2 signalling downregulated the basal, as well as the cAMP induced Il12rb2 expression, and blocking both further reduced Il12rb2 expression in TCR activated WT T cells at 36h, but cAMP still enhanced Il12rb2 expression over the basal level under these conditions (Fig. 2d).
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